Anticorps Polyclonal de lapin anti-CCAR2

CCAR2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 22638-1-AP

Synonymes

ccar2, DBC.1, DBC1, DBC-1, Deleted in breast cancer gene 1 protein, KIAA1967, NET35, p30 DBC, Protein KIAA1967

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Galerie de données de validation

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  WB analysis using 22638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22638-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22638-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22638-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22638-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22638-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22638-1-AP

  IP result of anti-CCAR2 (IP:22638-1-AP, 4ug; Detection:22638-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.

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  IHC staining of human breast cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.

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  IHC staining of human pancreas cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

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  IHC staining of human pancreas cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

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  IHC staining of human breast cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:100.

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  IHC staining of human breast cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:100.

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  IHC staining of human colon cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

• 

  IHC staining of human colon cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

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  IF Staining of HepG2 using 22638-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA1967 antibody 22638-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

22638-1-AP cible CCAR2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCAR2 Protéine recombinante Ag18374
• Nom complet: KIAA1967
• Masse moléculaire calculée: 923 aa, 103 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC018269
• Symbole du gène: CCAR2
• Identification du gène (NCBI): 57805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	CoIP,RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
mouse	WB, IF	Biochem Cell Biol; Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1.; Authors - Farshad Niri
human	WB,IHC,IP	Elife; Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression; Authors - Qiangqiang Liu
mouse	IF	Br J Pharmacol; AdipoRon promotes amyloid-β clearance through enhancing autophagy via nuclear GAPDH-induced sirtuin 1 activation in Alzheimer's disease; Authors - Fengjiao Sun
human	WB	Redox Biol; SIRT1 activated by AROS sensitizes glioma cells to ferroptosis via induction of NAD+ depletion-dependent activation of ATF3; Authors - Xi Chen