Anticorps Polyclonal de lapin anti-DARS2

• Phare
• Validé par KD/KO

DARS2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 13807-1-AP

Synonymes

Aspartate tRNA ligase, ASPRS, DARS2, LBSL, MT ASPRS, RP3 383J4.2

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Galerie de données de validation

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  WB analysis using 13807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13807-1-AP (DARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13807-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13807-1-AP (DARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13807-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13807-1-AP (DARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13807-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13807-1-AP (DARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13807-1-AP (DARS2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse cerebellum using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13807-1-AP (DARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13807-1-AP (DARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13807-1-AP (DARS2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13807-1-AP (DARS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13807-1-AP (DARS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13807-1-AP (DARS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13807-1-AP (DARS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 13807-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DARS2 antibody (13807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules U-937, tissu placentaire humain
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral humain, tissu de gliome humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 3 publications below

Informations sur le produit

13807-1-AP cible DARS2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DARS2 Protéine recombinante Ag4809
• Nom complet: aspartyl-tRNA synthetase 2, mitochondrial
• Masse moléculaire calculée: 645 aa, 74 kDa
• Poids moléculaire observé: 66-74 kDa
• Numéro d’acquisition GenBank: BC045173
• Symbole du gène: DARS2
• Identification du gène (NCBI): 55157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).

Publications

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial metabolism coordinates stage-specific repair processes in macrophages during wound healing.; Authors - Sebastian Willenborg
mouse	WB	Cell Metab; Tissue-Specific Loss of DARS2 Activates Stress Responses Independently of Respiratory Chain Deficiency in the Heart.; Authors - Sukru Anil Dogan
mouse	WB	EMBO Rep; Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt.; Authors - Dominic Seiferling ; KO Validated
human	WB,IHC	Am J Cancer Res; DARS2 is a prognostic biomarker and correlated with immune infiltrates and cuproptosis in lung adenocarcinoma; Authors - Xu-Sheng Liu ; KD Validated
mouse	WB	Int J Biochem Cell Biol; Mitochondrial oxidative phosphorylation is impaired in TALLYHO mice, a new obesity and type 2 diabetes animal model.; Authors - Caroline A Hunter
human,mouse	IHC,WB	Thorac Cancer; DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c-Myc signaling pathway; Authors - Tao Fang ; KD Validated