Anticorps Polyclonal de lapin anti-DAO

DAO Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 13273-1-AP

Synonymes

D amino acid oxidase, DAAO, DAMOX, DAO, OXDA

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Galerie de données de validation

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  WB analysis of mouse kidney using 13273-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13273-1-AP (DAO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13273-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13273-1-AP (DAO Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 13273-1-AP

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 13273-1-AP (DAO antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 13273-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13273-1-AP (DAO Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 13273-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13273-1-AP (DAO Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A549, tissu de cervelet de rat
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13273-1-AP cible DAO dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DAO Protéine recombinante Ag3973
• Nom complet: D-amino-acid oxidase
• Masse moléculaire calculée: 347 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC029057
• Symbole du gène: DAO
• Identification du gène (NCBI): 1610
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

D-amino acid oxidase (DAO),with MW of 39 kDa, is a flavoprotein which uses flavin adenine dinucleotide as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAO may play a role in the pathophysiology of schizophrenia and act as a detoxifying agent which removes D-amino acids that accumulate during aging. DAAO exists in solution as a mixture of monomer-dimer(80 kDa) and even higher molecular aggregates in equilibrium(170 kDa), depending on the enzyme concentration and forms(PMID: 11130179/PMID: 9210639).

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.; Authors - Danqing Xin
mouse	IF	Am J Physiol Cell Physiol; Functional role of histamine receptors in the renal cortical collecting duct cells.; Authors - Anastasia V Sudarikova
rat		Mol Cell Neurosci; d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production.; Authors - Takahiro Seki
human,rat	WB,IHC	Am J Physiol Renal Physiol; Renal Histaminergic System and Acute Effects of Histamine Receptor 2 Blockade on Renal Damage in the Dahl Salt-Sensitive Rat; Authors - Denisha R Spires

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (03-14-2023)

It does not work in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2, mouse liver, mouse kidney, mouse brain

DAO Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, mouse kidney, mouse brain (Cat no:13273-1-AP)