Anticorps Polyclonal de lapin anti-CstF-64

• Phare
• Validé par KD/KO

CstF-64 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 26825-1-AP

Synonymes

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2, CstF-64

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Galerie de données de validation

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  WB analysis of COLO 320 using 26825-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 26825-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26825-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26825-1-AP

  IP Result of anti-CstF-64 (IP:26825-1-AP, 4ug; Detection:26825-1-AP 1:300) with HeLa cells lysate 2900ug.

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  IHC staining of human breast cancer using 26825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26825-1-AP (CstF-64 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26825-1-AP (CstF-64 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26825-1-AP (CstF-64 antibody) at dilution of 1:150 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

26825-1-AP cible CstF-64 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CstF-64 Protéine recombinante Ag25254
• Nom complet: cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC017712
• Symbole du gène: CSTF2
• Identification du gène (NCBI): 1478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Publications

Species	Application	Title
human	CoIP	Neuron; Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.; Authors - Geneva R LaForce
human	IF	Biochem Biophys Res Commun; Cleavage stimulating factor 64 depletion mitigates cardiac fibrosis through alternative polyadenylation.; Authors - Rahul Neupane ; KD Validated
mouse	IHC	Front Oncol; CSTF2 Promotes Hepatocarcinogenesis and Hepatocellular Carcinoma Progression via Aerobic Glycolysis; Authors - Zhimin Chen
rat,mouse,human		iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min
human	WB	J Hepatocell Carcinoma; A Novel Gene Pair CSTF2/DPE2A Impacts Prognosis and Cell Cycle of Hepatocellular Carcinoma; Authors - Yangjin Huang