Anticorps Polyclonal de lapin anti-CYP7B1

CYP7B1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 24889-1-AP

Synonymes

CBAS3, CP7B, CYP7B1, Cytochrome P450 7B1, Oxysterol 7 alpha hydroxylase, SPG5A

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Galerie de données de validation

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  WB analysis of A431 using 24889-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24889-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24889-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 24889-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HEK-293 using 24889-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HEK-293 using 24889-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral humain, tissu hépatique de souris, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IHC: See 2 publications below

Informations sur le produit

24889-1-AP cible CYP7B1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP7B1 Protéine recombinante Ag19374
• Nom complet: cytochrome P450, family 7, subfamily B, polypeptide 1
• Masse moléculaire calculée: 506 aa, 58 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC136574
• Symbole du gène: CYP7B1
• Identification du gène (NCBI): 9420
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

Publications

Species	Application	Title
human,mouse,rat	WB	Free Radic Biol Med; ALDH2 deficiency exacerbates MCD-diet induced MASLD by modulating bile acid metabolism; Authors - Jun Luo
mouse	WB	Cell Physiol Biochem; Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.; Authors - Dandan Zhong
mouse	WB	Mol Nutr Food Res; The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation; Authors - Mengcheng Jin
mouse	WB	J Nutr Biochem; Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice.; Authors - Wenyun Li
mouse	WB	Biochem Pharmacol; Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.; Authors - Yanan Chen
mouse	WB	J Lipid Res; Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition.; Authors - Genta Kakiyama