Anticorps Polyclonal de lapin anti-CYP4F11

• Phare
• Validé par KD/KO

CYP4F11 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12286-1-AP

Synonymes

CYP4F11, CYPIVF11, Cytochrome P450 4F11

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Galerie de données de validation

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  WB analysis of human liver using 12286-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12286-1-AP

  WB result of CYP4F11 antibody (12286-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F11 transfected MCF-7 cells.

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  WB analysis of human kidney using 12286-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12286-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 12286-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12286-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12286-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CYP4F11 antibody (12286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MCF-7, tissu hépatique de souris, tissu hépatique humain, tissu rénal humain
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

12286-1-AP cible CYP4F11 dans les applications de WB, IHC, IF/ICC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP4F11 Protéine recombinante Ag2906
• Nom complet: cytochrome P450, family 4, subfamily F, polypeptide 11
• Masse moléculaire calculée: 524 aa, 60 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC016853
• Symbole du gène: CYP4F11
• Identification du gène (NCBI): 57834
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP4F11, also named as CYPIVF11, belongs to the cytochrome P450 family. It is the lone CYP4F/A enzyme that effectively oxidized 3-hydroxypalmitate. It is the predominant catalyst of this fatty acid oxidation reaction in human liver. CYP4F11 is one of the primary human epidermal keratinocytes. It is positively regulated by multiple signaling pathways in HaCaT keratinocytes, including RXR and JNK signaling pathways. (PMID:19812349)

Publications

Species	Application	Title
human	WB	Drug Metab Dispos; Gene regulation of CYP4F11 in human keratinocyte HaCaT cells.; Authors - Wang Ying Y
human	ChIP	Drug Metab Dispos; Regulation of cytochrome P450 4F11 by nuclear transcription factor-κB.; Authors - Bell Jordan C JC