Anticorps Polyclonal de lapin anti-CYP3A4

CYP3A4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

57

Avis (1)

N° de cat : 18227-1-AP

Synonymes

Albendazole monooxygenase, Albendazole sulfoxidase, CP33, CP34, CYP3A, CYP3A1, CYP3A11, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 3A4, Cytochrome P450 HLp, Cytochrome P450 NF 25, Cytochrome P450 PCN1, HLP, NF 25, Nifedipine oxidase, P450C3, P450PCN1, Quinine 3 monooxygenase

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Galerie de données de validation

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  WB analysis using 18227-1-AP

  Rat liver tissue was subjected to SDS PAGE followed by western blot with 18227-1-AP (CYP3A4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18227-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18227-1-AP (CYP3A4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours (mouse brain tissue and mouse colon tissue are negative control).

• 

  WB analysis of mouse liver using 18227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18227-1-AP (CYP3A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18227-1-AP (CYP3A4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 18227-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18227-1-AP (CYP3A4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 18227-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18227-1-AP (CYP3A4 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 39 publications below
• IHC: See 7 publications below
• IF: See 11 publications below

Informations sur le produit

18227-1-AP cible CYP3A4 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP3A4 Protéine recombinante Ag13074
• Nom complet: cytochrome P450, family 3, subfamily A, polypeptide 4
• Masse moléculaire calculée: 503 aa, 57 kDa
• Poids moléculaire observé: 51-57 kDa
• Numéro d’acquisition GenBank: BC101631
• Symbole du gène: CYP3A4
• Identification du gène (NCBI): 1576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984). Human CYP3A4 is homologous to five mouse P450 isoforms, namely, Cyp3a11, Cyp3a16, Cyp3a41a, Cyp3a41b, and Cyp3a44 (PMID: 22446519). And CYP3A4 is also the human homologue of rat CYP3A1 and CYP3A2 (PMID: 27546062, PMID: 32874920).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions; Authors - Shengjie Fan
mouse	IF	Hepatology; Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening.; Authors - Linjie Lv
human	IF	Acta Biomater; Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.; Authors - Kun Du
human	IF	ACS Appl Mater Interfaces; Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip; Authors - Xiaofang Du
monkey	IF	Int J Biol Sci; Hepatobiliary organoids differentiated from hiPSCs relieve cholestasis-induced liver fibrosis in nonhuman primates; Authors - Hongmei Li
human	IHC	Theranostics; Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.; Authors - Zhenyu Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (11-30-2022)

Good signal with this antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

CYP3A4 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:18227-1-AP)