Anticorps Polyclonal de lapin anti-CYP2W1

• Phare
• Validé par KD/KO

CYP2W1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12904-1-AP

Synonymes

CYP2W1, CYPIIW1, Cytochrome P450 2W1

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Galerie de données de validation

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  WB analysis of HEK-293 using 12904-1-AP

  WB result of CYP2W1 antibody (12904-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells.

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  WB analysis using 12904-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12904-1-AP (CYP2W1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12904-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12904-1-AP (CYP2W1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12904-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12904-1-AP (CYP2W1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12904-1-AP cible CYP2W1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP2W1 Protéine recombinante Ag3803
• Nom complet: cytochrome P450, family 2, subfamily W, polypeptide 1
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 45-54 kDa
• Numéro d’acquisition GenBank: BC025761
• Symbole du gène: CYP2W1
• Identification du gène (NCBI): 54905
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.

Publications

Species	Application	Title
human	WB	Pharmacol Rep; Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes.; Authors - Hanna Szaefer