Anticorps Polyclonal de lapin anti-CYP2D6/7

CYP2D6/7 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 17868-1-AP

Synonymes

CPD6, CYP2D, CYP2D@, CYP2D6, CYP2D6/7, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1

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Galerie de données de validation

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  WB analysis using 17868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17868-1-AP (CYP2D6/7 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17868-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17868-1-AP (CYP2D6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17868-1-AP (CYP2D6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17868-1-AP (CYP2D6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17868-1-AP (CYP2D6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17868-1-AP (CYP2D6 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

17868-1-AP cible CYP2D6/7 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP2D6/7 Protéine recombinante Ag12351
• Nom complet: cytochrome P450, family 2, subfamily D, polypeptide 6
• Masse moléculaire calculée: 497 aa, 56 kDa
• Poids moléculaire observé: 48-56 kDa
• Numéro d’acquisition GenBank: BC075023
• Symbole du gène: CYP2D6
• Identification du gène (NCBI): 1565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa. This antibody can recognize human CYP2D6/7, mouse CYP2D3/4/9/10/22/26, rat CYP2D1/3/4/10/26, due to the high homology.

Publications

Species	Application	Title
human	IHC	Theranostics; Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.; Authors - Zhenyu Wang
human	WB	Drug Des Devel Ther; Effects of Saikosaponin D on CYP1A2 and CYP2D6 in HepaRG Cells.; Authors - Hongfang Li
human	WB	Drug Metab Pharmacokinet; Cloning, sequence analysis, and tissue expression of marmoset paraoxonase 1.; Authors - Shotaro Uehara
human,mouse	WB	Xenobiotica; Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes.; Authors - Shotaro Uehara
rat	WB	Pharmazie; Silybin enhances the blood concentration of brexpiprazole in rats by downregulating expression of CYP3A4 and CYP2D6.; Authors - Congcong Yu
		J Adv Res; Gut microbiota and host Cyp450s co-contribute to pharmacokinetic variability in mice with non-alcoholic steatohepatitis: Effects vary from drug to drug; Authors - Jing Guo