Anticorps Polyclonal de lapin anti-CYP2C8/9/18/19

CYP2C8/9/18/19 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 16546-1-AP

Synonymes

CPC8, CYP2C8, CYP2C8/9/18/19, CYPIIC8, Cytochrome P450 2C8, Cytochrome P450 form 1, Cytochrome P450 IIC2, Cytochrome P450 MP 12, Cytochrome P450 MP 20, MP 12/MP 20, S mephenytoin 4 hydroxylase

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Galerie de données de validation

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  WB analysis using 16546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16546-1-AP (CYP2C8/9/18/19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16546-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16546-1-AP (CYP2C8/9/18/19 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16546-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16546-1-AP (CYP2C8/9/18/19 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 8 publications below

Informations sur le produit

16546-1-AP cible CYP2C8/9/18/19 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP2C8/9/18/19 Protéine recombinante Ag9898
• Nom complet: cytochrome P450, family 2, subfamily C, polypeptide 8
• Masse moléculaire calculée: 490 aa, 56 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC020596
• Symbole du gène: CYP2C8
• Identification du gène (NCBI): 1558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP2C8(Cytochrome P450 2C8) is also named as CYPIIC8, cytochrome P450 IIC2, cytochrome P450 MP-12, cytochrome P450 MP-20, cytochrome P450 form 1 and S-mephenytoin 4-hydroxylase. It belongs to the cytochrome P450 family. It is a monooxygenase involved in an NADPH-dependent electron transport pathway and a hepatic drug-metabolizing enzymes that oxidizes therapeutic drugs such as cerivastatin and endobiotics such as retinoic acid and arachidonic acid. It has 2 isoforms produced by alternative splicing. This antibody can recognize human CYP2C 8/9/18/19, mouse CYP2C 29/37/38/39/55/65/, rat CYP2C6/7/11/12/13/23 due to the high homology.

Publications

Species	Application	Title
mouse	WB	Cell Chem Biol; 3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.; Authors - Chenwen Shao
human	WB	Front Oncol; Prognostic Value and Correlation With Tumor Immune Infiltration of a Novel Metabolism-Related Gene Signature in Pancreatic Cancer.; Authors - Hui Chen
human	IHC	Front Oncol; Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.; Authors - Tingbo Ye
human	WB	Pharmaceutics; Assessing CYP2C8-Mediated Pharmaceutical Excipient-Drug Interaction Potential: A Case Study of Tween 80 and Cremophor EL-35.; Authors - Chengming Wen
rat	WB	Front Pharmacol; Mechanism of Paeoniflorin on ANIT-Induced Cholestatic Liver Injury Using Integrated Metabolomics and Network Pharmacology.; Authors - Lisheng Chen
human	IHC	BMC Cancer; Elevated 14,15- epoxyeicosatrienoic acid by increasing of cytochrome P450 2C8, 2C9 and 2J2 and decreasing of soluble epoxide hydrolase associated with aggressiveness of human breast cancer.; Authors - Xiaolong Wei