Anticorps Polyclonal de lapin anti-CYP27A1

CYP27A1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (1)

N° de cat : 14739-1-AP

Synonymes

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

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Galerie de données de validation

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  WB analysis of mouse liver using 14739-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14739-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

14739-1-AP cible CYP27A1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP27A1 Protéine recombinante Ag6408
• Nom complet: cytochrome P450, family 27, subfamily A, polypeptide 1
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC051851
• Symbole du gène: CYP27A1
• Identification du gène (NCBI): 1593
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.

Publications

Species	Application	Title
human	IHC	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng
	IHC	J Lipid Res; Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia.; Authors - Hiten D Mistry
rat	WB	Phytomedicine; Dehydrodiconiferyl alcohol, a lignan from Herpetospermum pedunculosum, alleviates cholestasis by activating pathways associated with the farnesoid X receptor.; Authors - Xiaodong Wei
rat	WB	Phytomedicine; Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism; Authors - FanCheng Meng
human,mouse	WB,IHC	Phytomedicine; Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.; Authors - Yu Fu
	WB	J Agric Food Chem; Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism.; Authors - Sisi Yan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

kishu (Verified Customer) (06-10-2021)

got good results in WB for human and rat liver cells

• Applications: Western Blot
• Cell Tissue Type: liver cells