Anticorps Polyclonal de lapin anti-CYP26B1

• Phare
• Validé par KD/KO

CYP26B1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (1)

N° de cat : 21555-1-AP

Synonymes

CYP26A2, CYP26B1, Cytochrome P450 26A2, Cytochrome P450 26B1, Cytochrome P450RAI 2, P450RAI 2, P450RAI2

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Galerie de données de validation

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  WB analysis using 21555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21555-1-AP (CYP26B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21555-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21555-1-AP (CYP26B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 21555-1-AP

  IP result of anti-CYP26B1 (IP:21555-1-AP, 4ug; Detection:21555-1-AP 1:500) with L02 cells lysate 2400ug.

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  IHC staining of human hepatocirrhosis using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21555-1-AP (CYP26B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21555-1-AP (CYP26B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21555-1-AP (CYP26B1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21555-1-AP (CYP26B1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP26B1 antibody (21555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, tissu cérébral humain
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

21555-1-AP cible CYP26B1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP26B1 Protéine recombinante Ag15368
• Nom complet: cytochrome P450, family 26, subfamily B, polypeptide 1
• Masse moléculaire calculée: 512 aa, 58 kDa
• Poids moléculaire observé: 49-58 kDa
• Numéro d’acquisition GenBank: BC069443
• Symbole du gène: CYP26B1
• Identification du gène (NCBI): 56603
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP26B1(Cytochrome P450 26B1) is involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation.It has 3 isoforms produced by alternative splicing.Cyp26b1 deficiency induces pronounced apoptosis in the developing limb and delayed chondrocyte maturation(PMID:15030763).Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA).

Publications

Species	Application	Title
human	WB	Nat Genet; Exome-wide analyses identify low-frequency variant in CYP26B1 and additional coding variants associated with esophageal squamous cell carcinoma.; Authors - Jiang Chang ; KD Validated
mouse	IHC	Brain Struct Funct; Activity-dependent and graded BACE1 expression in the olfactory epithelium is mediated by the retinoic acid metabolizing enzyme CYP26B1.; Authors - Hande Login
mouse	IF	J Neurosci; The Stimulus-Dependent Gradient of Cyp26B1+ Olfactory Sensory Neurons Is Necessary for the Functional Integrity of the Olfactory Sensory Map.; Authors - Hande Login
mouse	IF	J Neurosci; Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia.; Authors - Sofia Håglin
		Front Mol Neurosci; Transcriptomics of Environmental Enrichment Reveals a Role for Retinoic Acid Signaling in Addiction.; Authors - Yafang Zhang
human	WB	FASEB J; Dysregulated retinoic acid signaling in airway smooth muscle cells in asthma.; Authors - Amy E Defnet

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (02-06-2020)

The antibody stains a subset of proximal tubulues.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse kidney

CYP26B1 Antibody Immunofluorescence, validation (1:200 dilution) in mouse kidney (Cat no:21555-1-AP)