Anticorps Polyclonal de lapin anti-CYP19A1

• Phare
• Validé par KD/KO

CYP19A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 16554-1-AP

Synonymes

CYP19, CYAR, CPV1, Aromatase, ARO1

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Galerie de données de validation

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  WB analysis of A2780 using 16554-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16554-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16554-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of human placenta using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human placenta using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A2780, tissu ovarien de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, tissu cérébral de rat, tissu de gliome humain, tissu de muscle squelettique humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

16554-1-AP cible CYP19A1 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Oie
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP19A1 Protéine recombinante Ag9765
• Nom complet: cytochrome P450, family 19, subfamily A, polypeptide 1
• Masse moléculaire calculée: 20 kDa, 25 kDa, 58 kDa
• Poids moléculaire observé: 49-55 kDa
• Numéro d’acquisition GenBank: BC022896
• Symbole du gène: CYP19A1
• Identification du gène (NCBI): 1588
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).

Publications

Species	Application	Title
human	WB	Clin Transl Med; Treg deficiency-mediated TH 1 response causes human premature ovarian insufficiency through apoptosis and steroidogenesis dysfunction of granulosa cells.; Authors - Xue Jiao
mouse	WB	Sci Rep; Novel WT1 Missense Mutations in Han Chinese Women with Premature Ovarian Failure.; Authors - Huidan Wang
mouse, rat	WB	Sci Rep; Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application.; Authors - Jiansha Li ; KD Validated
human	WB,IHC	Fertil Steril; Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.; Authors - Miao Sun
rat	WB	Environ Pollut; Hormonal changes and folliculogenesis in female offspring of rats exposed to cadmium during gestation and lactation.; Authors - Zhiliang Li
mouse	WB	Endocrinology; The Immp2l Mutation Causes Ovarian Aging Through ROS-Wnt/β-Catenin-Estrogen Pathway: Preventive Effect of Melatonin.; Authors - Qing He