Anticorps Polyclonal de lapin anti-CYFIP1/2

CYFIP1/2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 16011-1-AP

Synonymes

CYFIP2, PIR121, p53-inducible protein 121, p53 inducible protein 121, KIAA1168

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Galerie de données de validation

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  WB analysis of human brain using 16011-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16011-1-AP

  IP result of anti-CYFIP1/2 (IP:16011-1-AP, 4ug; Detection:16011-1-AP 1:700) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 16011-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 16011-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CYFIP1/2 (16011-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de gliome humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

16011-1-AP cible CYFIP1/2 dans les applications de WB, IHC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYFIP1/2 Protéine recombinante Ag8749
• Nom complet: cytoplasmic FMR1 interacting protein 2
• Masse moléculaire calculée: 148 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC011762
• Symbole du gène: CYFIP2
• Identification du gène (NCBI): 26999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYFIP1/2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. CYFIP1 contains 1,253 amino acids and shares 88% sequence identity with CYFIP2. CYFIP1 and CYFIP2 are members of a highly conserved protein family and share approximately 99% sequence identity with their mouse orthologs. CYFIP1 has been shown to interact with the small GTPase RAC1, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and RAC1 localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts. This antibody can recognize both CYFIP1 and CYFIP2.

Publications

Species	Application	Title
human	WB	Dis Markers; Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis.; Authors - Nachmany Henny H
human	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lisa (Verified Customer) (03-17-2022)

The antibody works well for WB. Dilution 1:1000 in TBS-T 5% BSA.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuronal cells