Anticorps Polyclonal de lapin anti-NOX2

• Phare
• Validé par KD/KO

NOX2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF-P, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications

158

N° de cat : 19013-1-AP

Synonymes

CYBB, Heme-binding membrane glycoprotein gp91phox, EC:1.-.-.-, Cytochrome b-245 heavy chain, CGD91-phox

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Galerie de données de validation

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  WB analysis using 19013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 19013-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 19013-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 19013-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19013-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 19013-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NOX2 antibody (19013-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 19013-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 19013-1-AP (NOX2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 19013-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human NOX2 (19013-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules Jurkat, cellules Raji, plasma humain, tissu splénique de rat
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris, tissu d'amygdalite humain
• Résultats positifs en FC (Intra): cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 136 publications below
• IHC: See 16 publications below
• IF: See 15 publications below
• CoIP: See 1 publications below

Informations sur le produit

19013-1-AP cible NOX2 dans les applications de WB, IHC, IF-P, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, Lapin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOX2 Protéine recombinante Ag5536
• Nom complet: cytochrome b-245, beta polypeptide
• Masse moléculaire calculée: 577 aa, 65 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC032720
• Symbole du gène: NOX2
• Identification du gène (NCBI): 1536
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa(PMID: 17587483) to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781).

Publications

Species	Application	Title
mouse	WB	Circulation; Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury.; Authors - Yu Liming
mouse	WB,IHC	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
		Sci Adv; Sphingolipid biosynthesis is essential for metabolic rewiring during TH17 cell differentiation; Authors - Thiruvaimozhi Abimannan
human	WB,IF	Redox Biol; Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.; Authors - Hongtao Chen
pig	IF,WB	Redox Biol; Nox2 impairs VEGF-A-induced angiogenesis in placenta via mitochondrial ROS-STAT3 pathway.; Authors - Chengjun Hu ; KD Validated
rat	WB	Redox Biol; RND3 attenuates oxidative stress and vascular remodeling in spontaneously hypertensive rat via inhibiting ROCK1 signaling.; Authors - Nan Wu