Anticorps Polyclonal de lapin anti-CYB5R3

• Phare
• Validé par KD/KO

CYB5R3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 10894-1-AP

Synonymes

NADH-cytochrome b5 reductase 3, EC:1.6.2.2, Diaphorase-1, Diaphorase 1, DIA1

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Galerie de données de validation

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  WB analysis of HeLa using 10894-1-AP

  WB results of CYB5R3 antibody (10894-1-AP, 1:4000) with si-Control and si-CYB5R3 transfected HeLa cells.

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  WB analysis using 10894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10894-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10894-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10894-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 10894-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10894-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10894-1-AP

  IP result of anti-CYB5R3 (IP:10894-1-AP, 3ug; Detection:10894-1-AP 1:1000) with HepG2 cells lysate 6000ug.

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 10894-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (10894-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain, tissu placentaire humain, tissu pulmonaire humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 5 publications below
• IF: See 6 publications below

Informations sur le produit

10894-1-AP cible CYB5R3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYB5R3 Protéine recombinante Ag1339
• Nom complet: cytochrome b5 reductase 3
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC004821
• Symbole du gène: CYB5R3
• Identification du gène (NCBI): 1727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Publications

Species	Application	Title
mouse	WB	Sci Transl Med; Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes; Authors - Hitoshi Watanabe ; KO Validated
		J Cell Biol; Dia1-dependent adhesions are required by epithelial tissues to initiate invasion.; Authors - Tim B Fessenden
human,mouse	WB,IF	JCI Insight; CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling; Authors - Marta Bueno
rat	IHC	Brain Struct Funct; High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.; Authors - A K Samhan-Arias
mouse	IHC	Blood Adv; Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.; Authors - Katherine C Wood
mouse	WB	Free Radic Biol Med; Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3; Authors - Luz Marina Sánchez-Mendoza