Anticorps Polyclonal de lapin anti-CXorf56

CXorf56 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 24021-1-AP

Synonymes

CXorf56, STEEP, STING ER exit protein, UPF0428 protein CXorf56

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Galerie de données de validation

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  WB analysis of HeLa using 24021-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24021-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24021-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 24021-1-AP

  IP result of anti-CXorf56 (IP:24021-1-AP, 4ug; Detection:24021-1-AP 1:1000) with mouse kidney tissue lysate 8000ug.

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  IF Staining of HeLa using 24021-1-AP

  Immunofluorescent analysis of HeLa cells using 24021-1-AP (CXorf56 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

24021-1-AP cible CXorf56 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CXorf56 Protéine recombinante Ag19856
• Nom complet: chromosome X open reading frame 56
• Masse moléculaire calculée: 222 aa, 26 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC023506
• Symbole du gène: CXorf56
• Identification du gène (NCBI): 63932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CxORF56 (also known as STEEP) is an evolutionarily highly conserved protein whose function is unknown. Recently CxORF56 has been identified as a protein that interacts with STING, and it is essential for STING ER exit. CxORF56-dependent STING ER exit is essential for STING signaling in antiviral defense and in inflammatory diseases.

Publications

Species	Application	Title
human	WB	EBioMedicine; A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology.; Authors - Thaneas Prabakaran
monkey	WB	J Virol; The Papain-Like Protease of Porcine Reproductive and Respiratory Syndrome Virus Impedes STING Translocation from the Endoplasmic Reticulum to the Golgi Apparatus by Deubiquitinating STIM1; Authors - Feifei Diao
human	WB,IP	NPJ Breast Cancer; Inhibition of CXorf56 promotes PARP inhibitor-induced cytotoxicity in triple-negative breast cancer; Authors - Ying Zhu
human	WB	Nat Commun; Cholesterol-binding motifs in STING that control endoplasmic reticulum retention mediate anti-tumoral activity of cholesterol-lowering compounds; Authors - Bao-Cun Zhang