Anticorps Polyclonal de lapin anti-CXCR4

• Phare
• Validé par KD/KO

CXCR4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

46

Avis (1)

N° de cat : 11073-2-AP

Synonymes

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM

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Galerie de données de validation

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  WB analysis of rat spleen using 11073-2-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 11073-2-AP (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11073-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11073-2-AP (CXCR4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11073-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11073-2-AP (CXCR4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11073-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11073-2-AP (CXCR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11073-2-AP

  IP result of anti-CXCR4 (IP:11073-2-AP, 3ug; Detection:11073-2-AP 1:500) with HeLa cells lysate 1000ug.

• 

  IHC staining of human tonsillitis using 11073-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11073-2-AP (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11073-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11073-2-AP (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 11073-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11073-2-AP (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 11073-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11073-2-AP (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu splénique de rat, cellules HeLa, cellules Jurkat, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 42 publications below
• IHC: See 5 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Informations sur le produit

11073-2-AP cible CXCR4 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CXCR4 Protéine recombinante Ag1528
• Nom complet: chemokine (C-X-C motif) receptor 4
• Masse moléculaire calculée: 352 aa, 40 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC020968
• Symbole du gène: CXCR4
• Identification du gène (NCBI): 7852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

Publications

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	Nat Commun; N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.; Authors - Ri-Xin Chen
rat	WB	Acta Biomater; Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.; Authors - Shanshan Ma
mouse	WB	Theranostics; Bioengineering CXCR4-overexpressing cell membrane functionalized ROS-responsive nanotherapeutics for targeting cerebral ischemia-reperfusion injury.; Authors - Li Luo
rat	WB,IHC,IF	Biomater Sci; The effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegration; Authors - Chuan Zhou
mouse	WB	Brain Behav Immun; Cerebral ischemia increases bone marrow CD4(+)CD25(+)FoxP3(+) regulatory T cells in mice via signals from sympathetic nervous system.; Authors - Jianping Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hollie (Verified Customer) (07-05-2024)

Good detection of CXCR4. Some non-specific binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MT4 cells

CXCR4 Antibody Western Blot validation (1:1000 dilution) in MT4 cells (Cat no:11073-2-AP)

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