Anticorps Polyclonal de lapin anti-CXCL8/IL-8

CXCL8/IL-8 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 17038-1-AP

Synonymes

C X C motif chemokine 8, CXCL8, CXCL8/IL-8, Emoctakin, GCP 1, GCP/IL 8 protein I, GCP/IL 8 protein II, GCP/IL 8 protein III, GCP/IL 8 protein IV, GCP/IL 8 protein V, GCP/IL 8 protein VI, GCP1, IL 8, IL8, IL-8, interleukin 8, LYNAP, MDNCF

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Galerie de données de validation

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  WB analysis using 17038-1-AP

  PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17038-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17038-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: PMA, LPS and Brefeldin A treated THP-1 cells,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

17038-1-AP cible CXCL8/IL-8 dans les applications de IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CXCL8/IL-8 Protéine recombinante Ag10552
• Nom complet: interleukin 8
• Masse moléculaire calculée: 99 aa, 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC013615
• Symbole du gène: IL-8
• Identification du gène (NCBI): 3576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

Publications

Species	Application	Title
human		Mol Cancer; RIPK3 activation induces TRIM28 derepression in cancer cells and enhances the anti-tumor microenvironment.; Authors - Han-Hee Park
human		EMBO Rep; LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.; Authors - Xuedan Sun
human	IHC,IF	Cell Death Discov; Endometrial stromal cell ferroptosis promotes angiogenesis in endometriosis.; Authors - Guojing Li
human		Cell Death Dis; Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression.; Authors - Kezhou Zhu
	IHC	Int Immunopharmacol; Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis; Authors - Wei Wu
human	IHC	J Neurooncol; Radiotherapy induces an immediate inflammatory reaction in malignant glioma: a clinical microdialysis study.; Authors - Pedram Tabatabaei