Anticorps Polyclonal de lapin anti-CXCL14

• Phare
• Validé par KD/KO

CXCL14 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10468-1-AP

Synonymes

BMAC, bolekine, BRAK, C X C motif chemokine 14, Chemokine BRAK, CXCL14, Kec, KS1, MIP 2g, MIP2G, NJAC, PSEC0212, SCYB14, Small inducible cytokine B14

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Galerie de données de validation

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  IHC staining of human liver using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10468-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CXCL14 antibody (10468-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

10468-1-AP cible CXCL14 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CXCL14 Protéine recombinante Ag0747
• Nom complet: chemokine (C-X-C motif) ligand 14
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC003513
• Symbole du gène: CXCL14
• Identification du gène (NCBI): 9547
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CXCL 14 (C-X-C motif chemokine 14) is a small inducible cytokine, and may be a potent chemoattractant for neutrophils. CXCL14 possesses a destruction box (D-box) domain which acts as a recognition signal for degradation via the ubiquitin-proteasome pathway. CXCL14 is widely expressed in normal tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas without inflammatory stimuli. It is dispensable for dendritic cell function and localization within peripheral tissues. In localized prostate cancer, CXCL14 mRNA was significantly upregulated and positively correlated with Gleason score.

Publications

Species	Application	Title
human	IHC	Transl Res; Novel role of CXCL14 in modulating STAR expression in luteinized granulosa cells: implication for progesterone synthesis in PCOS patients.; Authors - Jia Qi
	IF	Dis Model Mech; Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.; Authors - Kyoung-In Cho
human		Front Pharmacol; Aberrant ROS Mediate Cell Cycle and Motility in Colorectal Cancer Cells Through an Oncogenic CXCL14 Signaling Pathway.; Authors - Jun Zeng ; KD Validated
human	IHC	Int Immunopharmacol; Comprehensive analysis of the expression and prognostic value of CXC chemokines in colorectal cancer.; Authors - Li Yu
human	IHC	Thyroid; Iodide-induced chemokines and genes related to immunological function in cultured human thyroid follicles in the presence of thyrotropin.; Authors - Yamazaki Kazuko K
human	IHC	J Transl Med; Chemokine CXCL14 is associated with prognosis in patients with colorectal carcinoma after curative resection.; Authors - Zeng Jun J ; KD Validated