Anticorps Polyclonal de lapin anti-CUEDC2

CUEDC2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 20123-1-AP

Synonymes

C10orf66, CUE domain containing 2, CUEDC2

---

Galerie de données de validation

• 

  WB analysis using 20123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20123-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20123-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 20123-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20123-1-AP

  IP result of anti-CUEDC2 (IP:20123-1-AP, 4ug; Detection:20123-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human ovary tumor using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens)..

• 

  IHC staining of human malignant melanoma using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens)..

• 

  IF Staining of HeLa using 20123-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUEDC2 antibody (20123-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules Jurkat, tissu cérébral de souris, tissu cérébral humain, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain, tissu de mélanome malin humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20123-1-AP cible CUEDC2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CUEDC2 Protéine recombinante Ag13847
• Nom complet: CUE domain containing 2
• Masse moléculaire calculée: 287 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000262
• Symbole du gène: CUEDC2
• Identification du gène (NCBI): 79004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif-containing protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis. It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers, such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) for dephosphorylation and inactivation by recruiting protein phosphatase (PP1), and thus repressed activation of the transcription factor NF-κB. Moreover, CUEDC2, is a key factor in endocrine resistance in breast cancer.