Anticorps Polyclonal de lapin anti-Cathepsin L

• Phare
• Validé par KD/KO

Cathepsin L Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10938-1-AP

Synonymes

Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, Major excreted protein, MEP

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Galerie de données de validation

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  WB analysis of A549 using 10938-1-AP

  WB result of Cathepsin L antibody (10938-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells.

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  WB analysis of A549 using 10938-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10938-1-AP (Cathepsin L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 10938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10938-1-AP (Cathepsin L Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 10938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10938-1-AP (Cathepsin L Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10938-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 10938-1-AP (Cathepsin L antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549,
• Résultats positifs en IHC: tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10938-1-AP cible Cathepsin L dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin L Protéine recombinante Ag1373
• Nom complet: cathepsin L1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 36-39 kDa, 29 kDa
• Numéro d’acquisition GenBank: BC012612
• Symbole du gène: Cathepsin L
• Identification du gène (NCBI): 1514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Publications

Species	Application	Title
human		Emerg Microbes Infect; GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus.; Authors - Danying Chen
human	IF	Front Microbiol; Cronobacter sakazakii ATCC 29544 Translocated Human Brain Microvascular Endothelial Cells via Endocytosis, Apoptosis Induction, and Disruption of Tight Junction.; Authors - Tong Jin
human	WB	J Cancer Res Clin Oncol; Expressions and significances of CTSL, the target of COVID-19 on GBM.; Authors - Qiang Dong
human	WB	Cancer Cell Int; Differential secretome of pancreatic cancer cells in serum-containing conditioned medium reveals CCT8 as a new biomarker of pancreatic cancer invasion and metastasis.; Authors - Peng Liu
human	IHC	Exp Eye Res; A proteomic approach towards understanding the pathogenesis of Mooren's ulcer.; Authors - Hao Chi
human	IHC	BMC Infect Dis; COVID-19: a fatal case of acute liver failure associated with SARS-CoV-2 infection in pre-existing liver cirrhosis.; Authors - Jana Ihlow