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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-Cathepsin K

Cathepsin K Polyclonal Antibody for IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 11239-1-AP

Synonymes

CTSK, EC:3.4.22.38, CTSO2, CTSO, catK



Applications testées

Résultats positifs en IPcellules ROS1728
Résultats positifs en IHCtissu de cancer du sein humain, tissu de cancer du poumon humain, tissu d'ostéosarcome humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu cérébral de souris,
Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

11239-1-AP cible Cathepsin K dans les applications de IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, poisson-zèbre, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Cathepsin K Protéine recombinante Ag1761
Nom complet cathepsin K
Masse moléculaire calculée 39 kDa
Poids moléculaire observé 29-36 kDa
Numéro d’acquisition GenBankBC016058
Symbole du gène Cathepsin K
Identification du gène (NCBI) 1513
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSK(cathepsin K), also named CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts (PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot (PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

Protocole

Product Specific Protocols
IHC protocol for Cathepsin K antibody 11239-1-APDownload protocol
IF protocol for Cathepsin K antibody 11239-1-APDownload protocol
IP protocol for Cathepsin K antibody 11239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Cell Biol

Circadian control of the secretory pathway maintains collagen homeostasis.

Authors - Joan Chang
mouseIHC,IF

Biomaterials

BMP2 immune complexes promote new bone formation by facilitating the direct contact between osteoclasts and osteoblasts.

Authors - Yamei Xu
mouse

ACS Appl Mater Interfaces

Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis

Authors - Wenzheng Liao
mouse

Br J Pharmacol

Andrographolide prevents bone loss via targeting ERRalpha-regulated metabolic adaption of osteoclastogenesis.

Authors - Tongling Huang
mouseIHC

Arthritis Rheum

Osteoarthritic change is delayed in a Ctsk-knockout mouse model of osteoarthritis.

Authors - Kozawa Eiji E
mouseIHC

Arthritis Rheum

Co-opting endogenous immunoglobulin for the regulation of inflammation and osteoclastogenesis in humans and mice.

Authors - MacLellan Lindsay M LM

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Isabel (Verified Customer) (07-03-2020)

Antibody worked really well to detect Cathepsin K in protein from mouse osteoclasts. Blot was really clean with no other bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Primary mouse osteoclasts
Cathepsin K Antibody Western Blot validation (1:500 dilution) in Primary mouse osteoclasts (Cat no:11239-1-AP)
FH

RICHA (Verified Customer) (10-09-2019)

Good but hard to get the signal

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Protein from mouse tail tendon
Cathepsin K Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:11239-1-AP)