Anticorps Polyclonal de lapin anti-Cathepsin F

• Phare
• Validé par KD/KO

Cathepsin F Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11055-1-AP

Synonymes

Cathepsin F, CATSF, CTSF

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Galerie de données de validation

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  IHC staining of human prostate cancer using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11055-1-AP (Cathepsin F antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse liver using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11055-1-AP (Cathepsin F antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11055-1-AP (Cathepsin F antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cardiaque de souris, tissu cardiaque humain, tissu de cancer de l'estomac humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Informations sur le produit

11055-1-AP cible Cathepsin F dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin F Protéine recombinante Ag1521
• Nom complet: cathepsin F
• Masse moléculaire calculée: 53 kDa and 34 kDa
• Numéro d’acquisition GenBank: BC011682
• Symbole du gène: Cathepsin F
• Identification du gène (NCBI): 8722
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSF(Cathepsin F) is a member of the papain family of cysteine proteases. These enzymes represent a major component of the lysosomal proteolytic system. They are synthesized as inactive precursors consisting of a signal sequence, a propeptide, and a catalytically active mature region. Cathepsins are routed to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The deduced 484-amino acid cathepsin F protein has a signal sequence and potential glycosylation sites.

Publications

Species	Application	Title
rat	IHC	Stem Cell Res Ther; Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression.; Authors - Chaoling Yao ; KD Validated
human	IHC	Brain Res Bull; Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes.; Authors - Saghizadeh Mehrnoosh M
human	IHC	Proc Natl Acad Sci U S A; A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB; Authors - Yuqi Lin
human	WB	Oncol Res; Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells; Authors - Ce Ji ; KD Validated