Anticorps Polyclonal de lapin anti-CTR9

CTR9 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 21264-1-AP

Synonymes

CTR9, KIAA0155, p150, p150TSP, SH2 domain binding protein 1, SH2BP1, TSBP

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Galerie de données de validation

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  WB analysis of Raji using 21264-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21264-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21264-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21264-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21264-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 21264-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 21264-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 21264-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 21264-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 21264-1-AP

  Immunofluorescent analysis of Hela cells, using CTR9 antibody 21264-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HEK-293, cellules HeLa, cellules L02, tissu pulmonaire de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

21264-1-AP cible CTR9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CTR9 Protéine recombinante Ag15690
• Nom complet: Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Masse moléculaire calculée: 1173 aa, 134 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: BC058914
• Symbole du gène: CTR9
• Identification du gène (NCBI): 9646
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Autophagy; The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.; Authors - Liangde Zheng
mouse	WB	Front Pharmacol; Tetrandrine Prevents Bone Loss in Ovariectomized Mice by Inhibiting RANKL-Induced Osteoclastogenesis.; Authors - Zeyuan Zhong
mouse	WB	Cell Death Dis; UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.; Authors - Gang Xiang
human	WB	Cell; SARS-CoV-2 variants evolve convergent strategies to remodel the host response; Authors - Mehdi Bouhaddou