Anticorps Polyclonal de lapin anti-CTPS2

CTPS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12852-1-AP

Synonymes

CTP synthase 2, CTP synthase II, CTP synthetase 2, CTPS2, UTP ammonia ligase 2

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Galerie de données de validation

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  WB analysis of mouse testis using 12852-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12852-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 12852-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12852-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12852-1-AP

  IP result of anti-CTPS2 (IP:12852-1-AP, 4ug; Detection:12852-1-AP 1:300) with HeLa cells lysate 1085 ug.

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  IHC staining of human prostate cancer using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HeLa, tissu de glande surrénale humain
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

12852-1-AP cible CTPS2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CTPS2 Protéine recombinante Ag3490
• Nom complet: CTP synthase II
• Masse moléculaire calculée: 586 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC034986
• Symbole du gène: CTPS2
• Identification du gène (NCBI): 56474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.

Publications

Species	Application	Title
human	IF	Int Rev Cell Mol Biol; Molecular cell biology and immunobiology of mammalian rod/ring structures.; Authors - Wendy C Carcamo
human	IF	J Cell Sci; Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.; Authors - S John Calise
human	WB	J Genet Genomics; Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction.; Authors - Gerson Dierley Keppeke