Anticorps Polyclonal de lapin anti-CTNS

CTNS Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13085-1-AP

Synonymes

CTNS, CTNS LSB, Cystinosin, cystinosis, nephropathic, PQLC4

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Galerie de données de validation

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  WB analysis using 13085-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13085-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13085-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 13085-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CTNS antibody (13085-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 13085-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 13085-1-AP (CTNS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13085-1-AP cible CTNS dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CTNS Protéine recombinante Ag3904
• Nom complet: cystinosis, nephropathic
• Masse moléculaire calculée: 400aa,45 kDa; 367aa,42 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC032850
• Symbole du gène: CTNS
• Identification du gène (NCBI): 1497
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The CTNS or cystinosin is a lysosomal membrane protein which comprises with seven transmembrane domains, a 128 amino acid N-terminal region bearing seven N-glycosylation sites and a cytosolic C-terminal GYDQL sorting motif. It functions as a H+-driven cystine transporter responsible for cystine export from lysosomes. The mutation of CTNS gene cause an inherited disorder, cystinosis, characterized by defective lysosomal efflux of cystine. This antibody was raised against the N-terminal region of human cystinosin. Two isoforms of CTNS exist due to alternative splicing events and both of them can be detected through this antibody.

Publications

Species	Application	Title
	WB	Biochem Biophys Res Commun; Reduced phosphate transport in the renal proximal tubule cells in cystinosis is due to decreased expression of transporters rather than an energy defect.; Authors - Mary L Taub
human	WB	Biochem Biophys Res Commun; Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.; Authors - Jong-Jer Lee
mouse	IF	J Immunol Methods; Characterization of a rat monoclonal antibody raised against ferroptotic cells.; Authors - Sho Kobayashi