Anticorps Polyclonal de lapin anti-CTGF

CTGF Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 23903-1-AP

Synonymes

CTGF

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Galerie de données de validation

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  WB analysis using 23903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23903-1-AP (CTGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 23903-1-AP

  IP result of anti-CTGF (IP:23903-1-AP, 4ug; Detection:23903-1-AP 1:1000) with mouse brain tissue lysate 1800 ug.

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  IHC staining of mouse brain using 23903-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23903-1-AP (CTGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 23903-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23903-1-AP (CTGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 23903-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23903-1-AP (CTGF Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human nephroblastoma using 23903-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 23903-1-AP (CTGF Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris,
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de souris, tissu de néphroblastome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

23903-1-AP cible CTGF dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CTGF Protéine recombinante Ag20985
• Nom complet: connective tissue growth factor
• Masse moléculaire calculée: 349 aa, 38 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC087839
• Symbole du gène: CTGF
• Identification du gène (NCBI): 1490
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. In western blotting, there are three different forms of CTGF reported: monomeric forms at approximately 36-38 kDa, homodimeric forms at approximately 70 kDa, and lower molecular mass fragment forms (PMID: 14592956).

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Aloe-emodin ameliorated MI-induced cardiac remodeling in mice via inhibiting TGF-β/SMAD signaling via up-regulating SMAD7; Authors - Jie Yu
mouse	WB,IHC	Biomater Sci; A high-density collagen xerogel thread prevents the progression of peritoneal fibrosis; Authors - Shigehisa Aoki
human,mouse	WB	Ecotoxicol Environ Saf; Polystyrene microplastic-induced extracellular vesicles cause kidney-related effects in the crosstalk between tubular cells and fibroblasts; Authors - Yung-Li Wang
human,rat	WB,IF	Stem Cells Int; hUMSCs Restore Uterine Function by Inhibiting Endometrial Fibrosis via Regulation of the MMP-9/TIMP-1 Ratio in CDDP-Induced Injury Rats; Authors - Yu Tang
rat	WB,IHC	J Cardiovasc Transl Res; Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.; Authors - Xinliang Zou
mouse	WB	Tissue Cell; Estrogen inhibits the differentiation of fibroblasts induced by high stiffness matrix by enhancing DNMT1 expression; Authors - Zhihan Zhao