Anticorps Polyclonal de lapin anti-CTBS

CTBS Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 12599-1-AP

Synonymes

chitobiase, di N acetyl, CTB, CTBS, Di N acetylchitobiase

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Galerie de données de validation

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  WB analysis of HeLa using 12599-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of SMMC-7721 using 12599-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse colon using 12599-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12599-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12599-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12599-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12599-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12599-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12599-1-AP (CTBS Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 12599-1-AP

  Immunofluorescent analysis of HepG2 cells, using CTBS antibody 12599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MCF-7, cellules SMMC-7721, tissu de côlon de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du foie humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12599-1-AP cible CTBS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CTBS Protéine recombinante Ag3228
• Nom complet: chitobiase, di-N-acetyl-
• Masse moléculaire calculée: 12 kDa, 43 kDa
• Poids moléculaire observé: 44-50 kDa
• Numéro d’acquisition GenBank: BC024007
• Symbole du gène: CTBS
• Identification du gène (NCBI): 1486
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTBS(Di-N-acetylchitobiase (chitobiase)) also named as CTB, is a lysosomal exoglycosidase that splits the GlcNAcβ-D-(l-4)GlcNAc chitobiose core of asparagine-linked glycoproteins. Chitobiase has been purified from human liver and kidney and rat liver. Both the human and rat enzymes are approximately 40-45 kDa and require a free reducing end GlcNAc for activity(PMID:1527079). This full length protein has a signal peptide and four glycosylation sites.