Anticorps Polyclonal de lapin anti-CTBP2

• Phare
• Validé par KD/KO

CTBP2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10346-1-AP

Synonymes

C-terminal-binding protein 2

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Galerie de données de validation

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  WB analysis using 10346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10346-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10346-1-AP

  IP result of anti-CTBP2 (IP:10346-1-AP, 4ug; Detection:10346-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IF Staining of HeLa using 10346-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTBP2 antibody (10346-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10346-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CTBP2 (10346-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Informations sur le produit

10346-1-AP cible CTBP2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris, Gerbille
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CTBP2 Protéine recombinante Ag0352
• Nom complet: C-terminal binding protein 2
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC002486
• Symbole du gène: CTBP2
• Identification du gène (NCBI): 1488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C-Terminal binding protein 2 (CTBP2) is a transcriptional repressor. It contains a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. This protein is thought to bind to the C-terminus of the adenovirus E1A proteins. Studies in mice suggested that this protein is involved in transcriptional repression. CTBP2 is expressed in all tissues tested, with a higher level of expression in the heart, skeletal muscle, and pancreas. The gene of CTBP2 is mapped to human chromosome 21q21.3.

Publications

Species	Application	Title
zebrafish	IF	PLoS Biol; Hair cell identity establishes labeled lines of directional mechanosensation.; Authors - Marta Lozano-Ortega
gerbil	IF	J Neurosci; Tonotopic variation in the calcium dependence of neurotransmitter release and vesicle pool replenishment at mammalian auditory ribbon synapses.; Authors - Johnson Stuart L SL
mouse	IF	Neural Plast; Specific Influences of Early Acoustic Environments on Cochlear Hair Cells in Postnatal Mice.; Authors - Aoshuang Chang
mouse	WB	Proteome Sci; Proteomic analysis of neonatal mouse hearts shows PKA functions as a cardiomyocyte replication regulator; Authors - Lizhi Hu
zebrafish	IF	Genet Mol Res; MicroRNA-200 family members are weakly expressed in the neurosensory epithelia of the developing zebrafish (Danio rerio) inner ear.; Authors - J T Du
human	WB,IHC,IF	J Clin Invest; The CTBP2-PCIF1 complex regulates m6Am modification of mRNA in head and neck squamous cell carcinoma; Authors - Kang Li ; KO Validated