Anticorps Polyclonal de lapin anti-CTBP1

• Phare
• Validé par KD/KO

CTBP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 10972-1-AP

Synonymes

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS

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Galerie de données de validation

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  WB analysis using 10972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10972-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10972-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10972-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10972-1-AP

  IP result of anti-CTBP1 (IP:10972-1-AP, 3ug; Detection:10972-1-AP 1:200) with mouse brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 10972-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10972-1-AP (CTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10972-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10972-1-AP (CTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (10972-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 10972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (10972-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 10972-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CTBP1 (10972-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules Jurkat, cellules NIH/3T3, cellules SH-SY5Y, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu de thymus de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

10972-1-AP cible CTBP1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CTBP1 Protéine recombinante Ag1425
• Nom complet: C-terminal binding protein 1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC011655
• Symbole du gène: CTBP1
• Identification du gène (NCBI): 1487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Publications

Species	Application	Title
mouse	WB	Nat Commun; CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.; Authors - Yongqing Liu
human	WB	Nat Commun; ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.; Authors - Lifang Li ; KD Validated
human	WB,IF	Oncogene; RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.; Authors - Yang Yu
human	WB	Mol Oncol; Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.; Authors - Xuejie Min
human	WB	Mol Carcinog; Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.; Authors - Kirthana Prabhakar ; KD Validated
mouse	IF	Curr Mol Med; Toosendanin Restrains Idiopathic Pulmonary Fibrosis by Inhibiting ZEB1/CTBP1 Interaction; Authors - Xingbin Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (02-18-2020)

This antibody worked perfectly. I had a specific band at ~48kD, which is at the predicted size of Ctbp1. Highly recommended for detecting Ctbp1 by western blots!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011