Anticorps Polyclonal de lapin anti-CST1

• Phare
• Validé par KD/KO

CST1 Polyclonal Antibody for WB, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IF, IHC, CoIP, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

13

N° de cat : 16025-1-AP

Synonymes

CST1, Cystain SA I, Cystatin 1, cystatin SN, Salivary cystatin SA 1

---

Galerie de données de validation

• 

  WB analysis of human saliva using 16025-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16025-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 16025-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16025-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  FC experiment of MCF-7 using 16025-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CST1 (16025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu salivaire humain, cellules COLO 320, tissu de glande surrénale humain, tissu testiculaire humain
• Résultats positifs en FC (Intra): cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 9 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16025-1-AP cible CST1 dans les applications de WB, IP, IF, IHC, CoIP, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CST1 Protéine recombinante Ag8862
• Nom complet: cystatin SN
• Masse moléculaire calculée: 141 aa, 16 kDa
• Poids moléculaire observé: 14-16 kDa
• Numéro d’acquisition GenBank: BC021225
• Symbole du gène: CST1
• Identification du gène (NCBI): 1469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
human	IHC,IF	Nat Commun; Integrated single-cell transcriptome analysis reveals heterogeneity of esophageal squamous cell carcinoma microenvironment.; Authors - Huy Q Dinh
human	WB	Cell Res; Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis.; Authors - Jin Lei L
human	WB,IHC,IF,IP	Oncogene; CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1; Authors - Dongbao Li ; KD Validated
human	WB	Mol Oncol; Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.; Authors - Go Itoh
human	IHC	Aging (Albany NY); Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.; Authors - Jing Han