Anticorps Polyclonal de lapin anti-CSPP1

• Phare
• Validé par KD/KO

CSPP1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 11931-1-AP

Synonymes

CSPP, CSPP1

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Galerie de données de validation

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  WB analysis of Jurkat using 11931-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11931-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 11931-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HEK-293 using 11931-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11931-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11931-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11931-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 11931-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CSPP1 antibody (11931-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Informations sur le produit

11931-1-AP cible CSPP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSPP1 Protéine recombinante Ag2521
• Nom complet: centrosome and spindle pole associated protein 1
• Masse moléculaire calculée: 141 kDa
• Poids moléculaire observé: 141 kDa
• Numéro d’acquisition GenBank: BC029445
• Symbole du gène: CSPP1
• Identification du gène (NCBI): 79848
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo

Publications

Species	Application	Title
human	WB,IF	Am J Hum Genet; Mutations in CSPP1 Cause Primary Cilia Abnormalities and Joubert Syndrome with or without Jeune Asphyxiating Thoracic Dystrophy.; Authors - Karina Tuz
human	WB,IF	Am J Hum Genet; Mutations in CSPP1 Lead to Classical Joubert Syndrome.; Authors - Naiara Akizu
human	WB	Cell Rep; A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.; Authors - Kari-Anne M Frikstad ; KO Validated
human	WB	EMBO Rep; CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.; Authors - Cecilia Aquino Perez ; KD Validated
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
human	IHC,IF	Br J Cancer; Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.; Authors - J Sternemalm ; KD Validated