Anticorps Polyclonal de lapin anti-CSNK2B-specific

• Phare
• Validé par KD/KO

CSNK2B-specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, FC (Intra), ELISA, IF

Conjugaison

Non conjugué

Publications

9

N° de cat : 20234-1-AP

Synonymes

CSNK2B, CSK2B, CK2N, CK2B, CK II beta

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Galerie de données de validation

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  WB analysis using 20234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20234-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20234-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 20234-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20234-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20234-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 20234-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CSNK2B-specific (20234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

20234-1-AP cible CSNK2B-specific dans les applications de WB, IHC, FC (Intra), ELISA, IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: casein kinase 2, beta polypeptide
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: NM_001320
• Symbole du gène: CSNK2B
• Identification du gène (NCBI): 1460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSNK2B, also named as CK2N, G5A nd CK II beta, belongs to the casein kinase 2 subunit beta family. CSNK2B participates in Wnt signaling. It plays a complex role in regulating the basal catalytic activity of the alpha subunit. The antibody is specific to CSNK2B.

Publications

Species	Application	Title
mouse	WB	Redox Biol; Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.; Authors - Ming Kong
mouse	WB	Phytomedicine; Spiropachysine A suppresses hepatocellular carcinoma proliferation by inducing methuosis in vitro and in vivo.; Authors - Yuan Fang
human	WB,IF	Front Mol Neurosci; Lovastatin Alleviates α-Synuclein Aggregation and Phosphorylation in Cellular Models of Synucleinopathy.; Authors - Lijun Dai
human	IHC, WB	J Cell Commun Signal; CSNK2B contributes to colorectal cancer cell proliferation by activating the mTOR signaling.; Authors - Shijun Yu ; KD Validated
mouse	WB,IF	Exp Cell Res; Oocyte-specific deletion of Gsα induces oxidative stress and deteriorates oocyte quality in mice.; Authors - Yue Xie ; KO Validated
human	WB	J Exp Clin Cancer Res; Overexpression of NELFE contributes to gastric cancer progression via Wnt/β-catenin signaling-mediated activation of CSNK2B expression.; Authors - Shijun Yu ; KD Validated

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C