Anticorps Polyclonal de lapin anti-CSK

CSK Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 17720-1-AP

Synonymes

C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK

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Galerie de données de validation

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  WB analysis using 17720-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 17720-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17720-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 17720-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17720-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 17720-1-AP

  IP result of anti-CSK (IP:17720-1-AP, 4ug; Detection:17720-1-AP 1:800) with HL-60 cells lysate 1000ug.

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  IHC staining of human lung using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 17720-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CSK antibody (17720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HL-60, cellules Jurkat, cellules K-562, tissu pulmonaire de rat
• Résultats positifs en IP: cellules HL-60
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

17720-1-AP cible CSK dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSK Protéine recombinante Ag12103
• Nom complet: c-src tyrosine kinase
• Masse moléculaire calculée: 450 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC106073
• Symbole du gène: CSK
• Identification du gène (NCBI): 1445
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).

Publications

Species	Application	Title
human	IHC	Cancer Res; miR-31 Displays Subtype Specificity in Lung Cancer.; Authors - Mackenzie L Davenport
human	WB	Cell Chem Biol; A strategy to assess the cellular activity of E3 ligase components against neo-substrates using electrophilic probes.; Authors - Benika J Pinch
human	WB	J Immunol; Systemic Lupus Erythematosus Immune Complexes Increase the Expression of SLAM Family Members CD319 (CRACC) and CD229 (LY-9) on Plasmacytoid Dendritic Cells and CD319 on CD56dim NK Cells.; Authors - Hagberg Niklas N
human	IP	Neoplasia; SUMOylation of Csk Negatively Modulates its Tumor Suppressor Function.; Authors - Nan Cui