Anticorps Polyclonal de lapin anti-Placental lactogen

Placental lactogen Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 16326-1-AP

Synonymes

Choriomammotropin, CS 2, CSB, CSH1, CSH2, hCS B, Lactogen, PL, Placental lactogen

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Galerie de données de validation

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  WB analysis using 16326-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 16326-1-AP (Placental lactogen antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16326-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16326-1-AP (Placental lactogen antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 16326-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16326-1-AP (Placental lactogen antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16326-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16326-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16326-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16326-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of human placenta using 16326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Placental lactogen antibody (16326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human placenta using 16326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Placental lactogen antibody (16326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu de côlon humain
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

16326-1-AP cible Placental lactogen dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Placental lactogen Protéine recombinante Ag9412
• Nom complet: chorionic somatomammotropin hormone 2
• Masse moléculaire calculée: 217 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC022044
• Symbole du gène: CSH2
• Identification du gène (NCBI): 1443
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The human growth hormone (GH) locus is comprised by two GH (GH1 and GH2) genes and three chorionic somatomammotropin (CSH1, CSH2 and CSH-L) genes. Placental lactogens (CSH1, CSH2) are produced in the mammalian placenta and are similar in structure and function to growth hormones. Together, placental lactogens and growth factors play an essential role to assure successful lactation after pregnancy. Placental lactogens also modify the metabolic state of the mother during pregnancy to supply energy to the fetus.

Publications

Species	Application	Title
	IHC	J Vet Med Sci; Localization of peptide hormones in the placentas of Bryde's (Balaenoptera brydei), sei (B. borealis), and common minke (B. acutorostrata) whales; Authors - Risa Aoki