Anticorps Polyclonal de lapin anti-GM-CSF

• Phare
• Validé par KD/KO

GM-CSF Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 17762-1-AP

Synonymes

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

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Galerie de données de validation

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  WB analysis using 17762-1-AP

  PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 17762-1-AP (GM-CSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17762-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17762-1-AP (GM-CSF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17762-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17762-1-AP (GM-CSF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 17762-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17762-1-AP (GM-CSF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 17762-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17762-1-AP (GM-CSF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: NK-92 cells,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Informations sur le produit

17762-1-AP cible GM-CSF dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GM-CSF Protéine recombinante Ag12142
• Nom complet: colony stimulating factor 2 (granulocyte-macrophage)
• Masse moléculaire calculée: 144 aa, 16 kDa
• Poids moléculaire observé: 15kDa,18 kDa ,22 kDa
• Numéro d’acquisition GenBank: BC108724
• Symbole du gène: GM-CSF
• Identification du gène (NCBI): 1437
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

Publications

Species	Application	Title
		J Hematol Oncol; FFAR2 expressing myeloid-derived suppressor cells drive cancer immunoevasion; Authors - Zeda Zhao
human	WB,IHC	J Exp Med; Tumoral EHF predicts the efficacy of anti-PD1 therapy in pancreatic ductal adenocarcinoma.; Authors - Jing Liu
mouse	IF	J Am Soc Nephrol; Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury.; Authors - Leyuan Xu
mouse	WB,IHC,IF	Acta Pharmacol Sin; Macrophages promote the transition from myocardial ischemia reperfusion injury to cardiac fibrosis in mice through GMCSF/CCL2/CCR2 and phenotype switching; Authors - Shi-Chun Shen
human,mouse	WB	Oncogene; CMAHP promotes metastasis by reducing ubiquitination of Snail and inducing angiogenesis via GM-CSF overexpression in gastric cancer.; Authors - Hsiang-Wei Huang
mouse	WB	Int J Mol Sci; Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice.; Authors - Tao Wang