Anticorps Polyclonal de lapin anti-Mu Crystallin

Mu Crystallin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12495-1-AP

Synonymes

CRYM, NADP-regulated thyroid-hormone-binding protein, Ketimine reductase mu-crystallin, EC:1.5.1.25, DFNA40

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Galerie de données de validation

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  WB analysis of Y79 using 12495-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12495-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12495-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12495-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12495-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12495-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human gliomas using 12495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human gliomas using 12495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 12495-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Mu Crystallin (12495-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules Jurkat, tissu cardiaque humain, tissu rénal humain
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de gliome humain,
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

12495-1-AP cible Mu Crystallin dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Mu Crystallin Protéine recombinante Ag3161
• Nom complet: crystallin, mu
• Masse moléculaire calculée: 314 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC018061
• Symbole du gène: Mu Crystallin
• Identification du gène (NCBI): 1428
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.

Publications

Species	Application	Title
mouse	IF	Dev Cell; Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.; Authors - John DeSisto
rat	WB	Endocr J; Follicular thyroglobulin enhances gene expression necessary for thyroid hormone secretion.; Authors - Yuko Ishido
rat	WB,IHC,IF	Neuropathology; Expression of CRYM in different rat organs during development and its decreased expression in degenerating pyramidal tracts in amyotrophic lateral sclerosis.; Authors - Reiji Hommyo

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C