Anticorps Polyclonal de lapin anti-Alpha B Crystallin

• Phare
• Validé par KD/KO

Alpha B Crystallin Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 15808-1-AP

Synonymes

Alpha B Crystallin, Alpha crystallin B chain, Alpha(B) crystallin, CRYA2, CRYAB, crystallin, alpha B, CTPP2, Heat shock protein beta 5, HSPB5, Rosenthal fiber component

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Galerie de données de validation

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  WB analysis using 15808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 15808-1-AP

  IP result of anti-Alpha B Crystallin (IP:15808-1-AP, 4ug; Detection:15808-1-AP 1:500) with U2OS cells lysate 1800 ug.

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  IHC staining of mouse eye using 15808-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15808-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15808-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 15808-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules U2OS, tissu cardiaque de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules U2OS,
• Résultats positifs en IHC: tissu oculaire de souris, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ELISA: See 1 publications below

Informations sur le produit

15808-1-AP cible Alpha B Crystallin dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha B Crystallin Protéine recombinante Ag8543
• Nom complet: crystallin, alpha B
• Masse moléculaire calculée: 175 aa, 20 kDa
• Poids moléculaire observé: 20-22 kDa
• Numéro d’acquisition GenBank: BC007008
• Symbole du gène: Alpha B Crystallin
• Identification du gène (NCBI): 1410
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.

Publications

Species	Application	Title
mouse	IF	Brief Bioinform; iSMNN: batch effect correction for single-cell RNA-seq data via iterative supervised mutual nearest neighbor refinement.; Authors - Yuchen Yang
human	WB	Oxid Med Cell Longev; Resveratrol Modulation of Protein Expression in parkin-Mutant Human Skin Fibroblasts: A Proteomic Approach.; Authors - Daniele Vergara
human,mouse	WB	Elife; Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.; Authors - Vasiliki-Ilya Gargareta
human	WB	Front Cell Dev Biol; A Novel Ferroptosis-Related Prognostic Signature Reveals Macrophage Infiltration and EMT Status in Bladder Cancer.; Authors - Yilin Yan ; KD Validated
fish	ELISA	Food Res Int; Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella).; Authors - Lihong Ge
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Deficiency of heat shock factor 4 promotes lens epithelial cell senescence through upregulating p21cip1 expression.; Authors - Xiukun Cui