Anticorps Polyclonal de lapin anti-TORC1/CRTC1

TORC1/CRTC1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 27647-1-AP

Synonymes

CRTC1, CREB-regulated transcription coactivator 1, KIAA0616, MECT1, TORC 1

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Galerie de données de validation

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  WB analysis of Neuro-2a using 27647-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27647-1-AP (CRTC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27647-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27647-1-AP (CRTC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 27647-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27647-1-AP (CRTC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27647-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27647-1-AP (CRTC1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules HEK-293T, cellules HeLa
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

27647-1-AP cible TORC1/CRTC1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TORC1/CRTC1 Protéine recombinante Ag26494
• Nom complet: CREB regulated transcription coactivator 1
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 68-80 kDa
• Numéro d’acquisition GenBank: BC017075
• Symbole du gène: CRTC1
• Identification du gène (NCBI): 23373
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRTC1, also named as Transducer of CREB protein 1, is a 634 amino acid protein, which belongs to the TORC family. CRTC1 is highly expressed in adult and fetal brain and is low expressed in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. CRTC1 localizes in Cytoplasm when is phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). it translocates to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels. CRTC1 as a Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. The calculated molecular weight of CRTC1 is 68 kDa, but the modified protein is about 70-75 kDa.

Publications

Species	Application	Title
human	WB	Autophagy; Keratinocyte autophagy enables the activation of keratinocytes and fibroblasts and facilitates wound healing.; Authors - Lei Qiang
rat	WB	Brain Res; The α1 adrenoceptor antagonist prazosin potentiates morphine induced conditioned place preference in rats; Authors - Wanyu Tu

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