Anticorps Polyclonal de lapin anti-CRNN

• Phare
• Validé par KD/KO

CRNN Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 11799-1-AP

Synonymes

58 kDa heat shock protein, C1orf10, cornulin, CRNN, DRC1, PDRC1, SEP53, Tumor related protein

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Galerie de données de validation

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  WB analysis of mouse brain using 11799-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11799-1-AP (CRNN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11799-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11799-1-AP (CRNN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human cervical cancer using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11799-1-AP (CRNN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11799-1-AP (CRNN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200.

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200.

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11799-1-AP (CRNN Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11799-1-AP (CRNN Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2
• Résultats positifs en IHC: tissu œsophagien humain, tissu cutané humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 9 publications below
• IF: See 1 publications below

Informations sur le produit

11799-1-AP cible CRNN dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRNN Protéine recombinante Ag2374
• Nom complet: cornulin
• Masse moléculaire calculée: 495 aa, 54 kDa
• Poids moléculaire observé: 70 kDa, 58 kDa
• Numéro d’acquisition GenBank: BC030807
• Symbole du gène: CRNN
• Identification du gène (NCBI): 49860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cornulin, also known as C1orf10, is a member of the "fused gene" family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. It may play a role in the mucosal/epithelial immune response and epidermal differentiation. Specific to squamous epithelia cells, cornulin is expressed in esophagus, primary keratinocytes, scalp skin, foreskin, cervix, larynx, and fetal bladder. It may be a marker of late epidermal differentiation, and is down-regulated in eczema.

Publications

Species	Application	Title
human,mouse	WB,IHC	J Invest Dermatol; Cornulin Is Induced in Psoriasis Lesions and Promotes Keratinocyte Proliferation via Phosphoinositide 3-Kinase/Akt Pathways.; Authors - Changji Li ; KD Validated
human	WB,IHC	J Proteomics; Plectin promotes migration and invasion of cancer cells and is a novel prognostic marker for head and neck squamous cell carcinoma.; Authors - Katada Koji K
human	IHC	Clin Oral Investig; Downregulation of CRNN gene and genomic instability at 1q21.3 in oral squamous cell carcinoma.; Authors - Iman Salahshourifar
human	IHC	J Thorac Cardiovasc Surg; Loss of CRNN expression is associated with advanced tumor stage and poor survival in patients with esophageal squamous cell carcinoma.; Authors - Hsu Po-Kuei PK
human	IHC	PLoS One; Expression of Ki-67, Cornulin and ISG15 in non-involved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC); Authors - Padmanabha Kumar Govindaraj
human	IHC	Ecancermedicalscience; Expression of cornulin in tongue squamous cell carcinoma.; Authors - Saira Saleem