Anticorps Polyclonal de lapin anti-CRISP3

• Phare
• Validé par KD/KO

CRISP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14847-1-AP

Synonymes

Aeg2, CRISP 3, CRISP3, CRS3, SGP28

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Galerie de données de validation

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  WB analysis using 14847-1-AP

  human saliva samples were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 14847-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 14847-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14847-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14847-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14847-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 14847-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14847-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14847-1-AP

  IP result of anti-CRISP3 (IP:14847-1-AP, 4ug; Detection:14847-1-AP 1:300) with human placenta tissue lysate 1520ug.

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  IHC staining of human prostate cancer using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: salive humain, cellules Jurkat, tissu de côlon de souris, tissu de thymus de souris, tissu ovarien de souris, tissu placentaire humain
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Informations sur le produit

14847-1-AP cible CRISP3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cysteine-rich secretory protein 3
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: NM_006061
• Symbole du gène: CRISP3
• Identification du gène (NCBI): 10321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRISP3, also known as SGP28, is a glycoprotein that belongs to a family of cysteine-rich secretory proteins (CRISPs). CRISP3 was originally discovered in human neutrophils, but transcripts are widely distributed in exocrine glands (salivary glands, pancreas, and prostate) and also found at lower levels in epididymis, ovary, thymus, and colon. CRISP3 is a potential biomarker for prostate cancer. This antibody can recognize both CRISP2 and CRISP3.

Publications

Species	Application	Title
human	IHC	J Natl Cancer Inst; Progesterone Receptor Isoform Ratio: A Breast Cancer Prognostic and Predictive Factor for Antiprogestin Responsiveness.; Authors - Paola A Rojas
human	IHC	J Hematol Oncol; Cysteine- rich secretory protein 3 (CRISP3), ERG and PTEN define a molecular subtype of prostate cancer with implication to patients' prognosis.; Authors - Samir Al Bashir
human	WB,IHC	Int J Biol Sci; Circular RNA hsa_circ_0003823 promotes the Tumor Progression, Metastasis and Apatinib Resistance of Esophageal Squamous Cell Carcinoma by miR-607/CRISP3 Axis; Authors - Yu-Ming Wang ; KD Validated
human	WB	J Dent Res; Proteome Analysis of Temporomandibular Joint with Disc Displacement; Authors - X Liu