Anticorps Polyclonal de lapin anti-CREB3

• Phare
• Validé par KD/KO

CREB3 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 11275-1-AP

Synonymes

CREB 3, CREB3, LUMAN, LZIP

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 11275-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11275-1-AP (CREB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11275-1-AP

  WB result of CREB3 antibody (11275-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB3 transfected HeLa cells.

• 

  IHC staining of human prostate cancer using 11275-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11275-1-AP (CREB3 antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of U2OS using 11275-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CREB3 antibody (11275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 2 publications below

Informations sur le produit

11275-1-AP cible CREB3 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CREB3 Protéine recombinante Ag1823
• Nom complet: cAMP responsive element binding protein 3
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 40-64 kDa
• Numéro d’acquisition GenBank: BC009402
• Symbole du gène: CREB3
• Identification du gène (NCBI): 10488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).

Publications

Species	Application	Title
human	WB	Sci Adv; Standalone or combinatorial phenylbutyrate therapy shows excellent antiviral activity and mimics CREB3 silencing.; Authors - Tejabhiram Yadavalli ; KD Validated
human	ChIP	Nat Commun; Golgi stress induces SIRT2 to counteract Shigella infection via defatty-acylation.; Authors - Miao Wang
human	WB	Int J Mol Sci; Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells.; Authors - Kentaro Oh-Hashi
human	WB,IHC	Cancers (Basel); miR766-3p and miR124-3p Dictate Drug Resistance and Clinical Outcome in HNSCC; Authors - Tomohiro Shibata
human	WB,IF,IP,ChIP	Front Mol Neurosci; LUMAN/CREB3 Plays a Dual Role in Stress Responses as a Cofactor of the Glucocorticoid Receptor and a Regulator of Secretion.; Authors - Jenna Penney
human		Carcinogenesis; Small leucine zipper protein promotes the metastasis of castration-resistant prostate cancer through transcriptional regulation of matrix metalloproteinase-13.; Authors - Suhyun Kim