Anticorps Polyclonal de lapin anti-CPSF4

• Phare
• Validé par KD/KO

CPSF4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 15023-1-AP

Synonymes

CPSF 30 kDa subunit, CPSF30, CPSF4, NAR, Neb 1, NEB1, No arches homolog

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Galerie de données de validation

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  WB analysis of HEK-293 using 15023-1-AP

  WB result of CPSF4 antibody (15023-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPSF4 transfected HEK-293 cells.

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  WB analysis of Jurkat using 15023-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15023-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15023-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15023-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15023-1-AP (CPSF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15023-1-AP

  IP result of anti-CPSF4 (IP:15023-1-AP, 3ug; Detection:15023-1-AP 1:400) with HeLa cells lysate 1000ug.

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  IHC staining of human endometrial cancer using 15023-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15023-1-AP (CPSF4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 15023-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15023-1-AP (CPSF4 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 15023-1-AP

  Immunofluorescent analysis of HepG2 cells, using CPSF4 antibody 15023-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

15023-1-AP cible CPSF4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPSF4 Protéine recombinante Ag7103
• Nom complet: cleavage and polyadenylation specific factor 4, 30kDa
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC003101
• Symbole du gène: CPSF4
• Identification du gène (NCBI): 10898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mature 3-prime ends of cellular mRNAs are producted by endonucleolytic cleavage of the primary transcripts, followed by polyadenylation of the upstream cleavage products. The cleavage and polyadenylation specificity factor (CPSF) has a essential role in pre-mRNA 3-prime end processing in that it is required in both the cleavage step and subsequent poly(A) addition [PMID: 9651582]. CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, whose other components are CPSF1, CPSF2, CPSF3 and FIP1L1.

Publications

Species	Application	Title
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB,IP	Mol Oncol; CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas.; Authors - Wangbing Chen ; KD Validated
human	WB,IHC,IF	Cancer Lett; Cleavage and polyadenylation specific factor 4 targets NF-κB/cyclooxygenase-2 signaling to promote lung cancer growth and progression.; Authors - Canhui Yi ; KD Validated
human	WB,IF	Mol Oncol; CREB-binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway.; Authors - Zhipeng Tang
pig	WB	Int J Biol Macromol; Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors.; Authors - Zhaohui Teng
human	WB, IF	Biochim Biophys Acta Mol Cell Res; Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT.; Authors - Qian Yang