Anticorps Polyclonal de lapin anti-CPSF3

• Phare
• Validé par KD/KO

CPSF3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 11609-1-AP

Synonymes

mRNA 3'-end-processing endonuclease CPSF-73, EC:3.1.27.-, CPSF73, CPSF 73 kDa subunit, CPSF 73

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Galerie de données de validation

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  WB analysis of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skin using 11609-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11609-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11609-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:700.

• 

  IHC staining of mouse kidney using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, tissu cérébral humain, tissu cutané humain
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu rénal de souris, tissu de cancer de la thyroïde humain, tissu de cervelet de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below

Informations sur le produit

11609-1-AP cible CPSF3 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPSF3 Protéine recombinante Ag2173
• Nom complet: cleavage and polyadenylation specific factor 3, 73kDa
• Masse moléculaire calculée: 684 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC020211
• Symbole du gène: CPSF3
• Identification du gène (NCBI): 51692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPSF3 (cleavage and polyadenylation specificity factor 3) is involved in human disease and is the putative target of benzoxaboroles in trypanosomatid parasites and Cryptosporidium infection. CPSF3 has also been associated with cancer malignancy in that CPSF3 induces cell cycle arrest in hepatocellular carcinoma and promotes lung adenocarcinoma cell proliferation and metastasis (PMID: 38718887). CPSF3 has been implicated in various cancers, including lung cancer, colorectal, prostate, and triple-negative breast cancers (PMID: 37627085).

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.; Authors - Kaili Luo ; KD Validated
human	WB	Cancers (Basel); CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma; Authors - Ying Huang
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
pig	WB	Cell Mol Life Sci; Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs; Authors - Xiaolong Yuan
human	WB	Cell Chem Biol; Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3; Authors - Ye Tao
human	WB	Cell Discov; RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation; Authors - Peng Lin ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fabienne (Verified Customer) (06-12-2024)

Works perfectly

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cell line

CPSF3 Antibody Western Blot validation (1:1000 dilution) in HeLa cell line (Cat no:11609-1-AP)