Anticorps Polyclonal de lapin anti-CPS1

• Phare
• Validé par KD/KO

CPS1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 18703-1-AP

Synonymes

CPS1, CPSase I

---

Galerie de données de validation

• 

  WB analysis of HeLa using 18703-1-AP

  WB result of CPS1 antibody (18703-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPS1 transfected HeLa cells.

• 

  WB analysis using 18703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18703-1-AP (CPS1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18703-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18703-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18703-1-AP (CPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18703-1-AP (CPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18703-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (18703-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 18703-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (18703-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

18703-1-AP cible CPS1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: carbamoyl-phosphate synthetase 1, mitochondrial
• Masse moléculaire calculée: 165 kDa
• Poids moléculaire observé: 140-165 kDa
• Numéro d’acquisition GenBank: BC140943
• Symbole du gène: CPS1
• Identification du gène (NCBI): 1373
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine. The antibody can recognize two isoforms of this gene around 165 kDa and 116 kDa.

Publications

Species	Application	Title
human	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala
mouse	IF	Nat Commun; Plasmodium sporozoite phospholipid scramblase interacts with mammalian carbamoyl-phosphate synthetase 1 to infect hepatocytes.; Authors - Sung-Jae Cha
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang
mouse	WB	iScience; Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions; Authors - Xinghong Guo
human	WB	J Cell Mol Med; Caspase recruitment domain family member 10 regulates carbamoyl phosphate synthase 1 and promotes cancer growth in bladder cancer cells.; Authors - Xi Liu ; KD Validated
human	WB	Front Cell Dev Biol; Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.; Authors - Changsen Bai ; KD Validated