Anticorps Polyclonal de lapin anti-CPEB4

CPEB4 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 25342-1-AP

Synonymes

CPE binding protein 4, CPE BP4, CPEB4, hCPEB 4, KIAA1673

---

Galerie de données de validation

• 

  WB analysis of SH-SY5Y using 25342-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25342-1-AP (CPEB4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 25342-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25342-1-AP (CPEB4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25342-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25342-1-AP (CPEB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 25342-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25342-1-AP (CPEB4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25342-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25342-1-AP (CPEB4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 25342-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CPEB4 antibody (25342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules A375, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

25342-1-AP cible CPEB4 dans les applications de WB, IHC, IF/ICC, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytoplasmic polyadenylation element binding protein 4
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: NM_030627
• Symbole du gène: CPEB4
• Identification du gène (NCBI): 80315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPEB4 also named as CPE binding protein 4 or hCPEB 4 is a 729 amino acid protein, which contains two RRM domains and belongs to the RRM CPEB family. CPEB4 involves in some forms of synaptic plasticity and regulate translation by binding to regulatory motifs of defined mRNA targets. CPEB1 and CPEB4 are differentially regulated by phase-specific kinases, generating the need of two sequential CPEB activities to sustain cytoplasmic polyadenylation during all the meiotic phases.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation.; Authors - Kai P Hoefig
human	WB	Front Cell Dev Biol; CPEB4 Inhibit Cell Proliferation via Upregulating p21 mRNA Stability in Renal Cell Carcinoma.; Authors - Jiehui Di
human	WB,IHC	J Transl Med; CircESRP1 enhances metastasis and epithelial-mesenchymal transition in endometrial cancer via the miR-874-3p/CPEB4 axis.; Authors - Rui Shi
human	WB	Technol Cancer Res Treat; miRNA-130a-3p/CPEB4 Axis Modulates Glioblastoma Growth and Progression; Authors - Hongchao Liu
mouse	WB	Acta Cir Bras; Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy.; Authors - Hui Zhang
mouse	WB	bioRxiv; Inhibition of CPEB3 ribozyme elevates CPEB3 protein expression and polyadenylation of its target mRNAs, and enhances object location memory; Authors - Claire C Chen