Anticorps Polyclonal de lapin anti-COX6B1

COX6B1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 11425-1-AP

Synonymes

COX VIb 1, COX6B, COX6B1, COXG

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Galerie de données de validation

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  WB analysis using 11425-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11425-1-AP (COX6B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 11425-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11425-1-AP (COX6B1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 11425-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using COX6B1 antibody (11425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Caco-2, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

11425-1-AP cible COX6B1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX6B1 Protéine recombinante Ag1984
• Nom complet: cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous)
• Masse moléculaire calculée: 80 aa, 10 kDa
• Poids moléculaire observé: 10-13 kDa
• Numéro d’acquisition GenBank: BC001015
• Symbole du gène: COX6B1
• Identification du gène (NCBI): 1340
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX6B1 (Cytochrome c oxidase subunit 6B1) is also named as COX6B and belongs to the cytochrome c oxidase subunit 6B family. It connects the two COX monomers into the physiological dimeric form. Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Publications

Species	Application	Title
human	WB	J Cell Biol; PDGFRβ translocates to the nucleus and regulates chromatin remodeling via TATA element-modifying factor 1.; Authors - Natalia Papadopoulos
human	WB	Hum Reprod; Lysine glutarylation in human sperm is associated with progressive motility.; Authors - Yi-Min Cheng
human		Hum Reprod; Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.; Authors - Yi-Min Cheng
human	WB	J Biol Chem; The cellular stress proteins CHCHD10 and MNRR1 (CHCHD2): Partners in mitochondrial and nuclear function and dysfunction.; Authors - Neeraja Purandare
human	WB	J Biol Chem; PMID: 20870728; Authors - Oliva Claudia R CR
rat	WB	Mol Med Rep; Overexpression of COX6B1 protects against I/R‑induced neuronal injury in rat hippocampal neurons.; Authors - Shan Yang