Anticorps Polyclonal de lapin anti-COQ9

• Phare
• Validé par KD/KO

COQ9 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 14874-1-AP

Synonymes

C16orf49, COQ9, PSEC0129

---

Galerie de données de validation

• 

  WB analysis using 14874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14874-1-AP (COQ9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14874-1-AP

  WB result of COQ9 antibody (14874-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COQ9 transfected HEK-293 cells.

• 

  IP experiment of HeLa using 14874-1-AP

  IP result of anti-COQ9 (IP:14874-1-AP, 3ug; Detection:14874-1-AP 1:500) with HeLa cells lysate 1840ug.

• 

  IHC staining of human liver cancer using 14874-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14874-1-AP (COQ9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14874-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14874-1-AP (COQ9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14874-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14874-1-AP (COQ9 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

14874-1-AP cible COQ9 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COQ9 Protéine recombinante Ag6645
• Nom complet: coenzyme Q9 homolog (S. cerevisiae)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC064946
• Symbole du gène: COQ9
• Identification du gène (NCBI): 57017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COQ9, also named as C16orf49, HSPC326, and PSEC0129, is a 318 amino acid protein, which belongs to the COQ9 family. COQ9 localizes in the Mitochondrion and is involved in the biosynthesis of coenzyme Q COQ9 has abilities to improve left ventricular performance and to reduce myocardial infarct size and cardiomyocyte apoptosis.

Publications

Species	Application	Title
rat	IF	J Clin Invest; COQ6 mutations in human patients produce nephrotic syndrome with sensorineural deafness.; Authors - Heeringa Saskia F SF
human	WB	J Am Soc Nephrol; ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment.; Authors - Eugen Widmeier
human	WB	J Med Genet; Human COQ4 deficiency: delineating the clinical, metabolic and neuroimaging phenotypes.; Authors - Lucia Laugwitz
human	WB	Front Physiol; Human COQ9 Rescues a coq9 Yeast Mutant by Enhancing Coenzyme Q Biosynthesis from 4-Hydroxybenzoic Acid and Stabilizing the CoQ-Synthome.; Authors - Cuiwen H He
human	WB	Mol Genet Metab; Detection of 6-demethoxyubiquinone in CoQ10 deficiency disorders: Insights into enzyme interactions and identification of potential therapeutics.; Authors - Diran Herebian ; KD Validated
human	IF	J Cell Biol; Coenzyme Q biosynthetic proteins assemble in a substrate-dependent manner into domains at ER-mitochondria contacts.; Authors - Kelly Subramanian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Ying (Verified Customer) (03-31-2021)

This antibody works well for our westerns of cultured mouse cell extracts.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse macrophage