Anticorps Polyclonal de lapin anti-COQ5

COQ5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 17453-1-AP

Synonymes

COQ5

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Galerie de données de validation

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  WB analysis of mouse heart using 17453-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17453-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17453-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17453-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

17453-1-AP cible COQ5 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COQ5 Protéine recombinante Ag10203
• Nom complet: coenzyme Q5 homolog, methyltransferase (S. cerevisiae)
• Masse moléculaire calculée: 327 aa, 37 kDa
• Poids moléculaire observé: 34.7 kDa
• Numéro d’acquisition GenBank: BC107874
• Symbole du gène: COQ5
• Identification du gène (NCBI): 84274
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB	J Exp Med; Implication of folate deficiency in CYP2U1 loss of function.; Authors - Claire Pujol
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Redox Biol; The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.; Authors - Pilar González-García
human	WB	J Am Soc Nephrol; ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment.; Authors - Eugen Widmeier
mouse	WB	EMBO Mol Med; The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene.; Authors - Marta Luna-Sánchez
mouse	WB	Sci Rep; Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis.; Authors - María Rodríguez-Hidalgo